The Effect of Colicin Activity on Escherichia Coli K-12 and Critical Endotoxic Membrane
Jasmine Zijin Cheng
Sir Winston Churchill
Floor Location : M 160 L
One of the major issues in modern medicine today is the emergence of superbugs, or infections caused by these hard to eradicate strains of microorganisms possessing the genes capable of withstanding multiple drugs (MDRS). The rate of mutation far surpasses our current speed and ability to manipulate existing drugs and/or manufacture new ones safe for use. One of the goals of this experiment was to find a solution to this problem with non-synthetic natural weapons created by microorganisms targeting microorganisms. The novelty of this idea is the potential of an endless supply of inexpensive and easy to manipulate sources naturally designed to combat the problem of stronger and more resilient strains.rnrn The objective of this project was to determine the capability of bacteriocins as natural bacteriostatic biotoxins to solve the problem of emerging bacterial resistance against synthetic antibiotics. Colicines, a by-product of selective strains of gram-negative Escherichia Coli bacteria containing the specific colicinogenic plasmid for the synthesis, immunity and release of these bacteriocins was the target of focus to determine also whether these bacteriocins were capable of acting upon similar strained gram-negative bacterial DNA and RNA. It is crucial to avoid direct lysis as select strains of gram negative bacteria have an LPS outer cell membrane, emulating an endotoxin which during stress response evokes the release of these toxins causing severe immune response in humans. Bacteria was harvested, purified and inoculated upon standard plates whereupon different quantities of Colicin was applied against a control without any external factor and incubated at 37 degrees Celsius for six hours and overnight.