Investigation into the novel proteins SGO_0707 and SGO_1487 in Streptococcus gordonii
Floor Location : S 195 H
Surface adhesion to tissues is essential for bacterial infection. Streptococcus gordonii is a gram-positive bacterial species that forms plaques in the oral cavity. Plaque formation is achieved through adhesion via various proteins that are located on the cell surface. This set of proteins allows S. gordonii to bind to each other, bind to tissues and establish infections that can lead to endocarditis. A better understanding of the proteins used by S. gordonii for adhesions could reveal new proteins that are used specifically by this bacteria, and provide targets for the prevention of infection. Two such proteins, SGO_0707 and SGO_1487, have been discovered (Davies et al.). While some cursory analysis has been carried out on these proteins, a more in-depth characterization may reveal more about the function of these proteins. Additionally, further characterization could guide new studies aimed at inhibiting the binding activity of these proteins. Through bioinformatic analysis using computer programs (BLAST, variety of tools on the Expasy server), these two proteins will be compared to other sequences in hopes of establishing relationships. Secondary and tertiary structures of the protein will be produced, and functional domains will be identified. Finally, to assess any unique functions, an analysis will be carried out on the unique stretch of sequence that defines the two proteins under study. The goal of this project will be to comprehensively characterize these proteins, after which an informed suggestion will be made on future avenues of research on these novel S. gordonii proteins.