Fish Egg-xaggeration? FINS and the Cytochrome "B"arcode
York House School
Floor Location : S 230 L
Several cases of food mislabeling have been found in restaurants these days, especially with delicacies such as "tuna", which is consumed and enjoyed by many. Some studies have shown that escolar in some cases has been used as a substitute, which has health risks outlined and specified by the CFIA. Mislabeling continues to be an issue as the producers need only to label their products using "market-accepted names", and are not required to identify the species of origin. Several methods of DNA based food authentication exists that use various types of technology. While still targeting sushi restaurants, I decided to test "nigiri" sushi labeled as "masago roe - smelt eggs" or "tobiko roe - flying fish eggs" as their eggs look similar in size, shape and colour while one is slightly more expensive than the other, which makes for an easy substitution. Using FINS, which stands for "Forensically Informative Nucleotide Sequencing" I was able to amplify and sequence the cytochrome B gene and determine the species of my purchased samples.