Natural Remedies: Do They Work?
Manveer Trehan, Farbod Nematifar
Sir Winston Churchill Secondary
Floor Location : M 004 H
In our experiment we tested the effectiveness of natural remedies specifically garlic, turmeric, ginger, and salt water in killing and preventing the growth of K-12 E. coli. Prior to performing the experiment we grew 3 dishes of E.coli and made the 5 natural remedy substances.
The first remedy was garlic, and we made the test substance by juicing 2 tablespoons of garlic using cheesecloth then adding the garlic itself and the juice into a bottle along with 2 tablespoons of water. We followed the same process for ginger however with turmeric and salt we simply combine 2 tablespoons of salt or turmeric with 2 tablespoons of water and we then mixed them in their individual bottles until they were dissolved. Lastly for our control we used basic distilled water. We also cut out 50 coffee filter circles with a diameter of cm as they would be used in place of filter paper for the experiment.
Once the E. coli had grown we took it out of the incubator and we proceeded inoculate it onto the Petri dishes, and we then closed the lid. We then dipped the coffee filters inside of the test liquid using a tweezers and we would proceed to drip off any excess before we placed it inside of the Petri dish. We put 3 coffee filters per Petri dish and we used 3 Petri dishes per substance. We then incubated the plates for 24 hours at 37 degrees upside down to prevent water from condensation to drip and contaminate the agar. Afterwards we took the dishes out and we measured the zone of inhibition for each substance. The area of the zone of inhibition included both the coffee filter and any surrounding area that had no bacterial growth.
Once we wrote down all of the data and we examined it and the obvious best natural remedy was garlic. . All of the results were quite similar and there were no outliers. Ginger and turmeric were able to create small zones of inhibition but they were not large enough to be deemed effective. Salt created absolutely no zone of inhibition but was as effective as the previous 2 because unlike turmeric and ginger allowed for smaller and fewer colonies of bacteria but it had no zone of inhibition.
In conclusion garlic is the most effective natural remedy in killing and preventing E. coli growth as it created the largest zone of inhibition and had the smallest and least amount of colonies inside its Petri dishes and the rest of the remedies were not strong enough to be deemed effective. We would like to thank the British Columbia Children's Hospital Research Institute for providing us with nutrient agar and other supplies and we would also like to thank Bruce Stephenson for providing us with supplies and excellent advice and help. We could not have done it without the help and support those individuals.