Aqua Bath? Genetic Engineering in Progress
Joyce Qi
Mulgrave School
Floor Location : J 116 D

The purpose of this lab is to determine how would plasmid insertion be different while the temperature shock change; therefore discover the general trend of what temperate shock does to affect plasmid insertion.

If the temperature of the water bath (temperature shock) measured by celcius increase until the most optimost, the plasmid would have a better chance to penetrate through the plasma membrane; therefore increase the chance of growing bacterial colonies with adapted fluorescent protein. In molecular level, high temperature would produce massive thermal energy, which can be transferred into kinetic energy that increased the collision rate between molecules. High thermodynamic energy might triggers the bi-phosphate, causing it to membrane to become more fluid. In cellular level, high temperature might cause the fatty acid tail of the phospholipid to become less rigid, and allow plasmids to freely diffuse through the plasma membrane into the cytoplasm. The foreign genome would include codons that can be translated for fluorescent protein synthesis. Theoretically, low temperature of the water bath might not be able to create the gap between phospholipid molecules and allow the plasmid to penetrate; high temperature would damage the peptidoglycan of cell wall or even denature the protein instead. The data collected in this experiment supported the hypothesis, and innovated a limiting factor to consider in future genetic engineering researches.

Overall, the most optimal temperature would have the highest chance of plasmid penetration, which strongly affects plasmid adaption.