The Effect of Vitamin B on Brine Shrimp Growth and Maturity
Nathaniel Tham
Sir Winston Churchill Secondary
Floor Location : J 206 D

This project is based on the brine shrimp (or Artemia), an invertebrate, and whether vitamin B added to the tank affects growth. I wondered if exposure to vitamin B leads to larger size and quicker maturity, even in lower level animals such as brine shrimp. I believed that if I added vitamin B solution to the tanks of brine shrimp, then, over the course of a few days, I would find higher growth rates in the brine shrimp than if I added none, because vitamin B increases nervous system and muscular development in other animals and in humans.

To conduct this experiment, I learned how to raise brine shrimp consistently, which took some trial and error as well as finding a way to measure their lengths. To raise brine shrimp, there are many conditions that are needed to keep the shrimp alive. I fed the brine shrimp pasteurized milk powder, specifically because of their low nutritional value, with no Vitamin B added. My method of growing the shrimp allowed me to see a difference in growth over 10 days.

Using thiamine as the main vitamin in the B-Complex added for my calculations, I was able to show over 2 trials that a lower “Half-B” concentration (0.21mg/L of thiamine, which is half the concentration of Vitamin B in the average human body) was the most successful, with the largest and most mature shrimp over 10 days. Growth in the tanks with “Full-B” dose (0.42mg/L thiamine, or full concentration of Vitamin B in the average human body) and where vitamin B was not added was not as good. In conclusion, the data from this experiment supports my hypothesis. It appears that vitamin B is necessary but there is an optimal level of vitamin B that is beneficial to the growth of brine shrimp.

My assumption therefore is that vitamin B is beneficial to most animals including invertebrates.