Fighting Fungi
Aishwarya Heran
Prince of Wales Secondary
Floor Location : M 127 H

Fungi are very common in the environment and people come in contact with fungal spores everyday without developing an infection. Certain types of fungi can cause fungal infections, and may be extremely harmful to the human host. It is important to treat fungal infections in an early stage and with products that are effective. Antifungal medications attempt to eliminate or reduce fungal growth. This project is an experiment to determine which topical antifungal agent works best to reduce yeast growth. I tested three different types of antifungal agents: clotrimazole, tolnaftate, and undecylenic acid. I chose to test these three antifungals because they work in different ways to reduce fungal growth, and are common over-the-counter medications. My hypothesis was that tolnaftate would be the most effective at reducing yeast growth, because it inhibits a rate-limiting enzyme in the synthesis of ergosterol. To test my hypothesis, Saccharomyces Cerevisiae (baker’s yeast) was treated with each antifungal agent and the amount of carbon dioxide the yeast produced was measured with a carbon dioxide collection apparatus. Yeast produces carbon dioxide when it is healthy and able to metabolize in an optimal manner. This project concluded that tolnaftate reduced yeast growth and carbon dioxide production the most. I believe that this experiment was quite successful because results were consistent through each trial and similar to published data.